Zeylanicobdella arugamensis, the marine leech from cultured crimson snapper (Lutjanus erythropterus), Jerejak Island, Penang, Malaysia
- Author:
Ravi Rajiv
;
Yahaya Shariman Zary
- Keywords:
Cytochrome oxidase subunit Ⅰ gene;
Zeylanicobdella arugamensis;
Scanning electron microscope;
Lutjanus erythropterus
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(5):473-477
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the prevalence,phylogenetics and DNA barcoding of Zeylanicobdella arugamensis (Z arugamensis) from crimson snapper (Lutjanus erythropterus),Jerejak Island,Penang,Malaysia.Methods:Experiment was conducted with 200 fish specimens of cultured Ltttjanus erythropterus from Jerejak Island,Penang,Peninsular Malaysia.The water temperature and length for each fish were measured prior to parasites examination.Next,the morphological identification of parasites was periormed.Genomic DNA from parasites was extracted for further molecular analysis,After PCR amplification,phylogenetic tree was constructed.The lowest Bayesian information criterion scores showed that the most compatible model is Tajima and Nei.Finally,data sets of cytochrome oxidase subunit Ⅰ gene sequence and trace file have been submitted to Barcode of Life Data System.Results:The prevalence rate of Z arugamensis was recorded to be 11.5％,and the intensity was 1.48.The low intensity was due to the water temperature recorded in this study (32.9-33.2 ℃).All the individuals of Z arugamensis recorded in this study showed a close relationship with species that were recorded in NCBI database (Z.arugamensis DQ414344,Aestabdella leiostomi DQ414305,Pterobdella amara DQ414334 and Cystobranchus meyeri DQ414315) but less relationship with Aestabdella abditovesiculata DQ414300.Finally,the DNA sequences submitted to Barcode of Life Data System in accordance to species have already obtained Barcode Index Number as BOLD:ACM3477.Conclusions:This study has provided an overview of sequence divergence at cytochrome oxidase subunit Ⅰ gene,DNA barcodes and parasite prevalence of Z arugamensis.
