Progress of liquid chromatography tandem mass spectrometry for the analysis of human steroid hormones
10.3760/cma.j.issn.1009-9158.2017.10.016
- VernacularTitle:液相色谱-串联质谱联用法检测人体类固醇激素的研究进展
- Author:
Weixuan LI
1
;
Qianqian LI
;
Shaojie YANG
;
Yan LI
Author Information
1. 528000,佛山市第一人民医院检验科
- Keywords:
Steroids;
Chromatography;
liquid;
Tandem mass spectrometry
- From:
Chinese Journal of Laboratory Medicine
2017;40(10):816-822
- CountryChina
- Language:Chinese
-
Abstract:
Steroid hormones are fat soluble molecules produced by cholesterol through a series of enzymatic catalysis.These low-level steroid hormones in body fluids generally play an important role in the regulation of metabolism and sexual function.Therefore,accurate quantification of these hormones is crucial for both diagnosis and treatment of endocrine disorder disease.At present, automated immunoassays are commonly used for the measurement of steroid hormone.However,only one analyte can be detected in one assay.More importantly, a lot of steroid hormones still cannot be detected by immunoassays due to the limitation of specific antibody.Liquid chromatography tandem mass spectrometry(LC-MS/MS)has high sensitivity and high specificity,and can simultaneously detect a variety of steroid hormones in one injection. LC-MS/MS has become a hot research topic in clinical research.
