Comparation of Pathogens in Infectious Gastroenteritis Cases Using Multiplex Ncleic Acid Amplification Testing and Conventional Laboratory Diagnostic Tools
10.3969/j.issn.1671-7414.2017.05.032
- VernacularTitle:多重核酸检测技术和传统检测方法对胃肠道感染性病原体检测的比较
- Author:
Jie YI
1
;
jie Hong XIE
;
jing Wen LIU
;
pei Yu ZHAO
;
chun Ying XU
Author Information
1. 中国医学科学院北京协和医院检验科
- Keywords:
infectious gastroenteritis diseases;
multiplex nucleic acid amplification testing;
conventional testing
- From:
Journal of Modern Laboratory Medicine
2017;32(5):118-121
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the performance of a commercial multiplex nucleic acid amplification test (MultiplexNAT) and the conventional microbiological testing for etiologic pathogens of gastroenteritis.Methods 135 stool specimens from 135 patients showing gastroenteritis symptoms were collected and detected by both the MultiplexNAT and the conventional testing.Results The detection rates of at least one potential etiologic agent was 81.5 ％ and 33.3％ by the MultiplexNAT and conventional testing,respectively.12 pathogens could be detected by the MultiplexNAT while 5 pathogens could be detected by the conventional testing.Of the negative samples from conventional testing,48.1％ were positive with the MultiplexNAT.Furthermore,31.1 ％ and none of the stool specimens showed coinfection by MultiplexNAT and conventional testing,respectively.Using MultiplexNAT,the positive detection rates of viruses were highest in the outpatient settings,emergency and inpatient settings,which were 15.6 ％,31.1 ％ and 3.7 ％ respectively.The overall proportion of pathogen-positive samples was higher for outpatient settings than for emergency and inpatient settings using both conventional testing and the MultiplexNAT.x2 test for paired data for statistical analysis:positive detection rates,coinfection positive detection rates and three settings positive detection rates using two methods was statistically significant respectively (x2 =45.57～58.887,P＜0.01).Conclusion The MultiplexNAT significantly has more postivie detection rates compared to the conventional testing,and could be a possible method in the diagnosis of infectious gastroenteritis diseases.
