Clinic Application of Levels of Serum β2-AR mRNA and NGF mRNA for Children with the Bronchial Asthma in Airway Remodeling
10.3969/j.issn.1671-7414.2017.05.019
- VernacularTitle:血清β2-AR mRNA和NGF mRNA水平检测在儿童支气管哮喘气道重塑中的应用价值
- Author:
Ping WANG
1
;
Xiong ZHANG
Author Information
1. 咸阳市第一人民医院儿科
- Keywords:
children asthma;
airway remodeling;
treatment;
beta2-ARmRNA;
NGFmRNA
- From:
Journal of Modern Laboratory Medicine
2017;32(5):69-73
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical application value of detection of serum beta 2 adrenergic receptor(beta 2-AR) mRNA and nerve tissue growth factor (NGF) mRNA in airway remodeling on children with bronchial asthma.Methods The clinical serum data of 33 cases of children with bronchial asthma in airway remodeling were selected,divided into the pre-treatment group and the post-treatment group,and selected 29 cases of children with respiratory diseases as the control group.The above research subjects were outpatients or inpatients from department of pediatrics in the First People's Hospital of Xianyang City from 2015.3 to 2017.3.The expression of serum beta 2-ARmRNA and NGFmRNA were detected by realtime fluorescence quantitative PCR (Real-time-PCR,RT-PCR).The relationship between the changes of serum 2-ARm-RNA and NGFmRNA and the occurrence of asthma remodeling was compared and analyzed.All the subjects were measured the inner diameter of the airway (L),diameter of the airway (D),the airway wall thickness (T) calculated by T=(D-L)/2,the airway lumen area (AI),total airway cross-sectional area (AO) with High Resolution Computerized Tomography in evaluation of airway remodeling of asthma through the above indexes.The clinical significance of serum beta 2-ARmRNA and NGFmRNA by receiver operating characteristic curve (ROC curve) was evaluated.Results The serum beta 2-ARmRNA and NGFmRNA expression in the post-treatment group were,respectively,up-regulation and down-regulation in comparison with the pre-treatment group,and there was significant difference (t =37.27,36.53,all P＜0.01),which in the post-treatment group were,respectively,down-regulation and up-regulation in comparison with the control group,and there was significant difference (t=7.95,12.67,all P＜0.01).The expression of erum beta 2-ARmRNA and NGFmRNA had a negative correlation in the remodeling group (r=-0.697,P＜0.01),which was associated obviously respectively with L,T and AI (r=0.775,-0.780,0.793,all P＜0.01),(r=-0.750,0.763,-0.779,all P＜0.01).The expression of erum beta 2-ARmRNA and NGFmRNA hada negative correlation in the pre-treatment group (r=-0.703,P＜0.01),which was associated obviously respectively with L,T and AI (r=0.782,-0.797,0.803,all P＜ 0.01),(r =-0.772,0.787,-0.809,all P ＜0.01).According to the statistical results of SPSS,ROC curve analysis showed that the expression levels of serum beta 2-ARmRNA and NGFmRNA in the remodeling group were 0.767 (95 ％ CI:0.658 ～ 0.876,P =0.000);0.744 (95 ％ CI:0.632 ～0.856,P=0.000).The sensitivity and specificity of beta 2-ARmRNA and NGF mRNA at the optimal critical value were 93.9 ％ and 96.2 ％,92.9 ％ and 95.7 ％.Conclusion The expression of serum beta 2-ARmRNA and NGFmRNA can monitor treatment of airway remodeling on children bronchial asthma,and estimate disease development,effectively control the progress of airway inflammation in reversing asthma disease progression to provide the basis for early intervention of children with bronchial asthma.
