Effect of Venetoclax on Tumor Associated Genes and Cytokines in Acute Myeloid Leukemia
10.3870/j.issn.1004-0781.2018.01.007
- VernacularTitle:维奈托克对急性髓系白血病肿瘤标志基因和炎症因子的影响
- Author:
Shuang ZHAO
1
;
Hongwei ZHAO
;
Wei MA
Author Information
1. 吉林大学第一医院药学部
- Keywords:
Venetoclax;
Leukemia;
myeloid;
acute;
Gene;
tumor associate;
Cytokines
- From:
Herald of Medicine
2018;37(1):31-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of venetoclax on RNA expression and cytokines release in lymphocytes isolated from acute myeloid leukemia(AML) patients. Methods MTT method was performed to study the apoptosis of AML cells and optimize the concentration of venetoclax.The gene markers expression and cytokines production before and after venetoclax treatment were examined by RT-PCR and ELISA methods,respectively. Results After chemotherapy,the expression of tumor associated inhibitory genes including BCL-2(P<0.05),SIRPα(P<0.05),CD47(P<0.05),PDL-1(P<0.05),PDL-2 (P<0.01),and EZH2(P<0.05) were significantly decreased when venetoclax reached 300 nmol·L-1.In addition,the expression levels of the activated molecules significantly increased,such as TRAIL(P<0.01) and TNF-α(P<0.01).The ELISA results showed that the release of human TNF-α and IFN-γ have significantly increased. Conclusion Venetoclax,which is a chemotherapeutic drug,can be used as tumor targeting drug.It can promote apoptosis in AML patients by regulating the genes expressions related to malignancy and cytokines production.
