Preparation and evaluation of dried blood spots control materials for steroids
10.3969/j.issn.1000-3606.2017.12.016
- VernacularTitle:干血滤纸片类固醇激素质控品制备及评价
- Author:
Xia ZHAN
1
;
Lianshu HAN
;
Jun YE
;
Wenjuan QIU
;
Xuefan GU
Author Information
1. 上海交通大学医学院附属新华医院 上海市儿科医学研究所小儿内分泌/遗传科 上海 200092
- Keywords:
dried blood spots;
steroid hormones;
quality control products;
tandem mass spectrometry
- From:
Journal of Clinical Pediatrics
2017;35(12):941-945
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the preparation method of dry blood spots (DBS) control materials for steroids used for internal quality control by liquid chromatography tandem mass spectrometry (LC-MS/MS). Methods Whole blood was collected and the blood cells and plasma were separated. The blood cells were washed by saline. The activated charcoal was added to the plasma. Standard substance was added to make different concentrations (low, medium, and high) of DBS control materials for steroids. The precision, accuracy, stability, and differences among different blood spots were detected and analyzed by LC-MS/MS. Results The inter-day precision and accuracy of DBS control materials for steroids were 2.4%-7.0% and 102.0%-111.0%, respectively, and the intra-day precision and accuracy were 5.1%-9.8% and 99.0%-114.8% respectively. The DBS control materials for steroids were stored for 5 months, and there was no difference among the different months (P>0.05). The coefficients of variation among different blood spots were small, 3.3%-8.2%. Conclusions The DBS control materials for steroids has good precision, accuracy and stability. The difference among different blood spots is small and meet the requirements of indoor quality control products. They can be used for the internal quality control in steroids detection by LC-MS/MS.
