Wnt3a regulates migration and adhesion of vascular smooth muscular cells via integrin-linked kinase
10.3969/j.issn.1671-8348.2017.36.004
- VernacularTitle:Wnt3a通过整合素连接激酶调节血管平滑肌细胞迁移和黏附
- Author:
Xiaolin WU
1
;
Rui ZHU
;
Bin LI
;
Wenwei LIU
;
Qing ZHOU
;
Yuqin ZHAO
Author Information
1. 湖北文理学院附属襄阳市中心医院心血管内科
- Keywords:
Wnt proteins;
muscle;
smooth;
vascular;
cell movement;
cell adhesion;
protein-serine-threonine kinases
- From:
Chongqing Medicine
2017;46(36):5049-5051
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and related mechanism of recombinant Wnt 3a protein on vascular smooth muscle cells(VSMCs) migration and adhesion .Methods Primary rat VSMCs were cultured .The experiment was divided into the Wnt3a group and control group .The VSMC migration ability was detected by Transwell experiment .And the ability of VSMC ad-hesion to extracelluar matrix was detected by the celluar matrix adhesion assay .The protein expression levels of β-catenin ,phos-phor-β-catenin (Ser675) ,GSK-3β,phosphor-GSK-3β(Ser9) and integrin-linked kinase(ILK) in VSMCs were detected by Western blot and analyzed .Results The number of VSMCs migration in the Wnt3a group was significantly higher than that in the control group (P<0 .05) .Compared with the control group ,the number of VSMCs adhesion to collagen Ⅰ and the optical density value in the Wnt3a group were significantly increased (P<0 .05) ,and the protein expression levels of β-catenin ,Ser675 ,GSK-3β,Ser9 and ILK in the Wnt3a group were significantly up-regulated (P<0 .05) .Conclusion Wnt3a can regulate VSMCs migration and adhesion by ILK .
