Detection of Atropine Sulfate by Surface Enhanced Raman Spectroscopy
- VernacularTitle:利用表面增强拉曼光谱技术检测硫酸阿托品
- Author:
Siqingaowa HAN
1
;
Xuanyu SHA
;
Hang ZHAO
;
Lin BAO
;
Wuliji HASI
Author Information
1. 内蒙古民族大学附属医院蒙医口腔科 内蒙古通辽028007
- Keywords:
Surface enhanced Raman spectroscopy;
Atropine sulfate;
Silver sol;
Atractylodes Lancea ( Thunb. ) DC.
- From:
China Pharmacist
2017;20(12):2277-2281
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a surface enhanced Raman spectroscopy ( SERS) technique for the determination of atropine sulfate. Methods:The Raman peaks of atropine sulfate molecules were classified by theoretical calculations and experimental tests, and the 1002 cm-1 Raman peaks were selected as the characteristic peaks for the quantitative analysis. Results:The detection limit of atropine sulfate in aqueous solution was below 0. 5 μg·ml-1 . The relationship between the intensity of characteristic peaks at 1002 cm-1 and the concentration of aqueous solution was linear within the range of 1-8 μg ml-1 with the linear correlation coefficient r of 0. 9839. The recovery rates of 2, 5 and 7μg ml-1 were measured, which were 97. 1%-109. 8%. The average recovery was 103. 3%, and the RSD was 4. 5% (n=9). At the same time, the stability of the method among the batches was tested, and the relative standard deviation was 5. 7 %. In addition, the atractylodes rhizome samples containing atropine sulfate were detected,and the characteristic peaks still could be detected at 1002 cm-1 . Conclusion:The method is rapid, accurate and nondestructive with easy operation, which can be used for the detection of atropine sulfate.
