Differences between infiltration of mononuclear-macrophages and neutrophils during Leptospira inter-rogans infection and underlying mechanisms
10.3760/cma.j.issn.0254-5101.2017.10.003
- VernacularTitle:问号钩端螺旋体感染过程中单核-巨噬细胞和中性粒细胞浸润差异及机制
- Author:
Xu CHEN
1
;
310058 杭州,浙江大学医学院病原生物学系
;
Ying LIU
;
Ming WANG
;
Jie YAN
;
peng Guang TANG
;
Shijun LI
Author Information
1. 550004 贵阳,贵州省疾病预防控制中心;310058 杭州,浙江大学医学院病原生物学系
- Keywords:
Leptospira interrogans;
Mononuclear-macrophage;
Neutrophil;
Infiltration;
Chemo-kine
- From:
Chinese Journal of Microbiology and Immunology
2017;37(10):734-739
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the differences between infiltration of mononuclear-macro-phages and neutrophils during Leptospira interrogans (L.interrogans) infection and the underlying mecha-nisms. Methods Histological changes in mouse lung, liver and kidney tissues were detected using hema-toxylin and eosin (HE) staining following infection of C3H/HeJ mice with L.interrogans serovar Lai strain 56601. Infiltration of peripheral blood-derived CD11b+mononuclear-macrophages and Ly6G+neutrophils in lung,liver and kidney tissues collected form L.interrogans-infected C3H/HeJ mice was detected with immu-nohistochemistry. Levels of mononuclear-macrophage chemokines and neutrophil chemokines in serum sam-ples of L.interrogans-infected mice were detected with chemokine detection microarray. Results Lung,liv-er and kidney tissue samples collected from L. interrogans-infected C3H/HeJ mice presented typical his-topathological changes of leptospirosis, such as inflammatory cell infiltration in these tissues, pulmonary hemorrhage,extensive hepatocyte necrosis and serious nephrohemia. Results of immunohistochemical stai-ning showed that a large number of peripheral blood-derived CD11b+mononuclear-macrophages were presen-ted in lung,liver and kidney tissues of L.interrogans-infected mice, but few neutrophils could be found in these tissues. The mouse chemokine detection microarray confirmed that the levels of mononuclear-macro-phage chemokines (I-309,MCP-1,MCP-5,MIP-1α and RANTES) in serum samples of L.interrogans-in-fected C3H/HeJ mice were significantly increased during infection (P<0.05), but the neutrophil chemokines(KC,LIX and MIP-2) analyzed in this study were not notably increased (P>0.05). Conclu-sion Mononuclear-macrophages rather than neutrophils are the major infiltrating phagocytes during L.inter-rogans infection and play a crucial role in the elimination of Leptospira invasion.
