Establishment and Preliminary Application of the Gene Chip Detection Method of Human Pneumosis Virus
10.3969/j.issn.1671-7414.2017.06.005
- VernacularTitle:人偏肺病毒基因芯片检测方法的建立及初步应用
- Author:
ling Ai LIU
1
;
yan Shu LIU
;
sheng Ai LIU
;
Jun LIU
;
yun Cai ZHU
;
hua Long GUO
Author Information
1. 深圳市龙华区人民医院检验科
- Keywords:
human pneumovirus;
gene chip;
conventional reverse transcriptase polymerase chain reaction;
set up;
application
- From:
Journal of Modern Laboratory Medicine
2017;32(6):19-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish human metapneumovirus(hMPV)nucleotide rapid,specific and sensitive gene chip detec-tion method,and provide effective diagnostic methods for hMPV detection and diagnosis in shenzhen area.Methods The flu-orescence PCR primer of hMPV was designed for the highly conservative regional gene sequence of hMPV virus.Application of array probe design software designer 4.20 hMPV oligonucleotide detection probe design,oligonucleotide probe sample points to aldehyde slides on the preparation of hMPV gene chip,and parallel compared with conventional reverse tran-scriptase-polymerase chain reaction(RT-PCR),the sensitivity,specificity and repeatability,and was used to evaluate the clinical applicability of the samples.Results hMPV gene chip method to detect hMPV specificity was 96.23%(230/239), the sensitivity was 2.0×101/μl,linear range was 2.0×101~2.0×107/μl,and the repeatability was good.Initial tests in shenzhen area 300 clinical specimens on a nasopharyngeal swab,gene chip method detection rate was 20.3%(61/300),sig-nificantly higher than the conventional RT-PCR method 9.7%(29/300),the difference was statistically significant between the sensitivity of two methods(χ2=39.205,P<0.05).The results were consistent in two methods(kappa=0.360 7).Con-clusion Established hMPV microarray assay,gene chip method to detect hMPV has high specificity and sensitivity,wide linear range and detection.The popularity of hMPV monitoring for laboratory provides a new detecting technology and early diagnosis.
