Investigation on Drug Resistance Threshold of Newly Found HIV Infected Persons in Shaanxi
10.3969/j.issn.1671-7414.2017.06.039
- VernacularTitle:陕西省新发现艾滋病感染者HIV耐药警戒线调查研究
- Author:
Qiang REN
1
;
lin Yu FU
;
Hua LI
;
fan Yang ZOU
;
yan Meng ZHANG
;
Chao ZHANG
;
hui Wen CHANG
Author Information
1. 陕西省疾病预防控制中心
- Keywords:
human immunodeficiency virus;
drug resistance gene;
drug resistance threshold;
subtype
- From:
Journal of Modern Laboratory Medicine
2017;32(6):136-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the resistance gene sequences of newly untreated HIV infected people,and to understand the prevalence and epidemic characteristics of HIV drug-resistant strains in Shaanxi Province.Methods According to the rec-ommended by the World Health Organization HIV drug resisitance threshold(HIVDR-TS),sera from 47 newly diagnosed HIV infected patients aged 16~25 years were analyzed.In-house determination of pol gene partial sequence,using online CPR software to determine whether there was transmission of resistant mutants,and submitted to Stanford University in the United States online HIV drug resistance database for the extent of the drug resistance analysis,the phylogenetic tree was constructed using mega 5.0 software,analysis of subtypes.Results No spread drug mutations were found in 47 samples,but there were 14 samples that were found to have associated with resistance gene mutation sites,including the RT region T69N and E138A,V179D/E mutations,PR A71T/V,L10I/V mutation sites and in subtype CRF01_B a like this in the site K103R,with V179E and there was a synergistic effect,resulting in accordance with efavirenz(EFV)and nevirapine(NVP) drugs such as moderately resistant.The mutations found in the remaining 13 samples would lead to a low degree of resist-ance to some of the drugs,and there was no clinical significance.Conclusion The prevalence rate of HIV resistant strains in Shaanxi province was <5%,which be longed to the low epidemic level.It is not necessary to detect drug resistance before individual treatment,and it is necessary to carry out the investigation of drug resistance.
