Effects of chemokine Fractalkine on proliferation and invasion of human pancreatic cancer cell lines by regulating IL-6/STAT3 signal pathway
10.3969/j.issn.1671-8348.2017.28.002
- VernacularTitle:趋化因子Fractalkine通过调控IL-6/STAT3信号通路对人胰腺癌细胞株增殖和侵袭的影响研究
- Author:
Haiyang LI
1
;
Liya HUANG
;
Yang WU
;
Hui LIU
Author Information
1. 宁夏医科大学,银川750004
- Keywords:
chemokine Fractalkine;
transfection;
pancreatic cancer cell lines;
interleukin 6;
STAT3
- From:
Chongqing Medicine
2017;46(28):3893-3895,3899
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of chemokine Fractalkine(FKN) on the proliferation and invasion of human pancreatic cancer cell lines PANC-1 and SW-1990 by regulating IL-6/STAT3 signal pathway.Methods Adenovirus served as the vector to construct and synthesizing FKN-small interfering RNA(siRNA),then which was transfected into PANC-1 and SW-1990.The proliferation and invasion ability of cells was determined by CCK-8 assay and Transwell assay.Expression of FKN,IL-6 and STAT3 protein and mRNA was detected by Western blot and RT-qPCR.Results After transfecting FKN-siRNA for 24 h,the absorbance values(A value) in the PANC-1 and SW-1990 groups had no significant changes,the A value at 48,72 h in the FKN-siRNA group was significantly higher than that in the control group and FKN-siRNA negative group (P＜0.05).After transfecting FKN-siRNA,the cellular invasive ability in the PANC-1 and SW-1990 FKN-siRNA group was significantly stronger than that in the control group and FKN-siRNA negative group(P＜0.05).After transfecting FKN-siRNA in cell lines PANC-1 and SW-1990,compared with the control group and FKN-siRNA negative group,the FKN protein and mRNA expression in the FKN-siRNA group was significantly decreased(P＜0.05),while IL-6 and STAT3 protein and mRNA expression was significantly increased(P＜0.05).Conclusion Chemokine FKN might play the inhibiting effect on the biological activity of pancreatic cancer cells by regulating IL-6/ STAT3 signal pathway.
