Mechanism of Salidroside on Regulating Disorder of Glucose and Lipid Metabolism of Primary Mouse Hepatocytes
10.3870/j.issn.1004-0781.2017.11.011
- VernacularTitle:红景天苷改善小鼠原代肝细胞糖脂代谢紊乱机制
- Author:
Xinru ZHANG
1
;
Wei ZHAO
;
Dongxue WANG
;
Xiujuan FU
Author Information
1. 吉林大学第二医院药品管理部
- Keywords:
Salidroside;
Hepatocytes;
Glucose metabolic disorder;
Lipid metabolic disorder
- From:
Herald of Medicine
2017;36(11):1260-1263
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of salidroside on regulating glucose and lipid metabolism in high sugar high fat induced primary mouse hepatocytes,and to explore the molecular mechanism. Methods Primary hepatocytes were extracted from mice and cultured, then divided into normal control group, model control group ( high sugar high fat induced) ,and salidroside group(high sugar high fat induced and salidroside treated).Model control group and salidroside group were cultured in a high sugar and fatty medium with the optimal concentration of salidroside for 24 h. The glucose consumption, glycogen, and triglyceride concentration were detected, and key proteins of glucose and lipid metabolism were detected by RT-PCR in all three groups. Results Compared with model control group, the glucose consumption and glycogen concentration were both increased ( P<0. 05 ) in salidroside group ( P<0. 05 ) , triglyceride concentration were decreased ( P<0. 05 ) , the mRNA level of gluconeogenesis key protein ( G6Pase and PEPCK) and lipid synthesis key protein ( SREBP-1c, FAS-1, DGAT-2) mRNA expression level were increased (P<0.05),and the level of lipid oxidation key protein (CPT-1α) was decreased (P<0.05). Conclusion Salidroside can reduce insulin resistance and correct glucose and lipid metabolic disorder in high sugar high fat induced primary mouse hepatocytes.
