Content Determination and Safety Evaluation of Aflatoxins B1 , B2 , G1 and G2 in Shugan Pills
- VernacularTitle:舒肝丸中的黄曲霉毒素B1、B2、G1、G2的含量测定及安全性评价
- Author:
Yanan ZHOU
1
;
Yongli LIU
;
Hao YUAN
Author Information
1. 河北省药品检验研究 石家庄050011
- Keywords:
Shugan pills;
Aflatoxin B1;
B2;
G1 and G2;
Quality control
- From:
China Pharmacist
2017;20(10):1869-1871
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine aflatoxin B1 , B2 , G1 and G2 in Shugan pills by HPLC and evaluate their safety. Methods:The product was analyzed by HPLC using a Shiseido C18 column(150 mm × 4. 6 mm,5 μm)with methanol-acetonitrile-water (10 :30 :60) as the mobile phase. An ultraviolet lamp was derivative at 254 nm, and the fluorescence detection was performed with the excitation wavelength at 360 nm and the emission wavelength at 450nm. The samples from nation-wide market were detected by the method. Re-sults:Good linear relationships were obtained within the range of 10-50 pg for aflatoxin B1 and G1 , and 3-15 pg for aflatoxin B2 and G2 . The recovery of aflatoxin B1 , B2 , G1 and G2 was 89. 9%,87. 9%,86. 1% and 87. 1%,respectively with all the RSD below 3. 0%. The results of all samples were satisfactory, and the qualification rate of the samples was 100%, indicating that the samples were safe. Conclusion:The developed method is convenient and accurate, which can be applied in the quantitative determination of Shugan pills.
