The Detection of Epstein-Barr Virus and Expression of bcl-2 and p53 Proteins in the Extranodal Malignant Lymphoma of the Head and Neck.
- Author:
Chang Hoon BAI
1
;
Si Youn SONG
;
Yong Dae KIM
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, Korea. ydkim@med.yu.ac.kr
- Publication Type:Original Article
- Keywords:
Lymphoma;
Epstein-Barr virus;
bcl-2;
p53
- MeSH:
Carcinogenesis;
Head*;
Herpesvirus 4, Human*;
Humans;
In Situ Hybridization;
Lymphoma*;
Lymphoma, T-Cell, Peripheral;
Neck*;
Oncogenes
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2007;50(4):335-340
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Although the cause of the malignant lymphoma has not been understood entirely, viruses and oncogenes have been implicated as probable causative factors. Particularly, Epstein-Barr virus (EBV), the over-expression of bcl-2 and the mutant p53 seem to be closely related to lymphomatogenesis. The purpose of this study was to investigate the relationship between EBV, bcl-2 and p53 proteins in the development of human extranodal malignant lymphoma in the head and neck. SUBJECTS AND METHOD: Specimens were obtained from 40 patients diagnosed as the extranodal malignant lymphoma in the head and neck region. Paraffin-embedded tumor tissues were analyzed by in situ hybridization for Epstein-Barr virus encoded RNA(EBER) and by immunohistochemical technique for bcl-2 and p53 proteins. RESULTS: In EBER and bcl-2 protein-positive group, there were no significant differences in the pathologic types. However, the angiocentric lymphoma type (85.7%) and the peripheral T cell lymphoma unspecified large cell type (83.3%) were identified significantly in the p53 protein positive group (p<0.05). Expression rate of EBER, bcl-2 and p53 protein were 50%, 42.5% and 53.5%, respectively. In the EBER-positive group (20 cases), bcl-2 protein was detected in 9 cases (45.0%) and p53 protein in 11 cases (55.0%). In bcl-2 protein-positive group (17 case), p53 protein was detected in 9 cases (52.9%). There were no significant difference among these data (p>0.05). CONCLUSION: These results suggest that EBV, bcl-2 and p53 protein play a role in the tumorigenesis of the human extranodal malignant lymphoma, independently.
