The Mechanism of Methacholine Induced Ciliostimulation: The Relationship with Cyclooxygenase Pathway and Nitric Oxide.
- Author:
Woo Sub SHIM
1
;
Tae Bin WON
;
Chae Seo RHEE
;
Chul Hee LEE
;
Yang Gi MIN
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, Korea. csrhee@snu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Mucociliary clearance;
Methacholine chloride;
Nitric oxide;
Cyclooxygenase
- MeSH:
Acetylcholine;
Animals;
Diclofenac;
Epithelium;
Methacholine Chloride*;
Mucociliary Clearance;
Mucous Membrane;
Nitric Oxide*;
Prostaglandin-Endoperoxide Synthases*;
Rats
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2006;49(5):517-521
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Mucociliary movement of respiratory ciliated epithelium is one of important protective mechanisms in the upper respiratory airway. It is well known that methacholine induces a ciliostimulation. This study designed to investigate the regulatory mechanism of methacholine induced ciliostimulation. MATERIALS AND METHOD: We investigated in vitro effect of methacholine, a beta-methyl ester of acetylcholine, and nitric oxide on ciliary beat frequency (CBF) of nasal septal mucosa of SD rat. CBF was measured using a video computerized analysis system. RESULTS: Methacholine (10(-6) mol/L) increased CBF upto 6 hours with a maximum increase of 44%. Diclofenac inhibited the ciliostimulatory effect of methacholine completely. LNAME inhibited the increase in CBF by methacholine significantly (p<.05). CONCLUSION: The results suggest that ciliostimulatory effects by methacholine in airway epithelium involves prostaglandin pathway and may be regulated by nitric oxide.
