A Study of Susceptibility between Allergic Rhinitis and Tumor Necrosis Factor Gene Polymorphism Study in Korean.
- Author:
Jae Hoon LEE
1
;
Tae Wook CHOI
;
Jung Hun LEE
;
Ha Min JEONG
;
Jung Youl MIN
;
Jeong Joong KIM
Author Information
1. Department of Otolaryngology, Institute of Wonkwang Medical Science, College of medicine, Wonkwang University, School of medicine, Iksan, Korea. coolnose@wmc.wonkwang.ac.kr
- Publication Type:Original Article
- Keywords:
Tumor necrosis factor-alpha;
Allergic rhinitis;
Korean;
Polymorphism
- MeSH:
Alleles;
Cell Count;
Eosinophils;
Genotype;
Guanine;
Humans;
Immunoassay;
Immunoglobulin E;
Inflammation;
Major Histocompatibility Complex;
Rhinitis*;
Tumor Necrosis Factor-alpha*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2005;48(9):1107-1110
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Tumor necrosis factor-alpha (TNF-alpha) is recognized as a proinflammatory host alert cytokine that is synthesized early during inflammation, and elevations of this cytokine have been reported in patients with allergic rhinitis. The TNF-alpha gene is located on chromosome 6p within the class III region of the major histocompatibility complex. The most widely studied polymorphism within the TNF-alpha gene involves a guanine (G)/adenine (A) substitution at position -308. We therefore tested whether TNF-alpha -308G/A polymorphism are associated with allergic rhinitis in a Korean population. SUBJECTS AND METHOD: Blood samples for genetic analysis were obtained from 191 individuals with allergic rhinitis and from 192 healthy subjects without atopic diseases. Polymerase chain reaction-based assay for TNF-alpha -308G/A was used for genotyping. Serum total IgE levels were determined by using the immunoassay. Eosinophil values were determined by eosinophil numbers per total cell numbers per microliter. RESULTS: There were no differences in the frequencies of the genotypes and alleles of TNF-alpha -308G/A in the controls and patients(p>0.05). Blood eosinophil count and total serum IgE levels were not statistically different in the genotypes of TNF-alpha -308G/A in allergic rhinitis(p>0.05). CONCLUSION: We thought that the TNF-alpha -308G/A polymorphism is unlikely to be an important marker for predisposition to allergic rhinitis. A further study involving a larger Korean population might be in need.
