The Effect of Combination Therapy with Radiation and Selective Cyclooxygenase-2 Inhibitor on Xenografted Human Oral Cavity Squamous Cell Carcinoma in Nude Mice.
- Author:
Jun Hee KIM
1
;
Sung Yun PARK
;
Hee Jong KIM
;
Ki Bum KIM
;
Hoon Shik YANG
;
Kyu Suk LEE
;
Ho Jin LEE
;
Young Hun JI
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Chung-Ang University, Seoul, Korea. cauent@hanafos.com
- Publication Type:Original Article
- Keywords:
Radiation therapy;
Cyclooxygenase 2 inhibitors;
Squamous cell carcinoma;
Radiation sensitizing agents
- MeSH:
Animals;
Apoptosis;
Carcinoma, Squamous Cell*;
Cyclooxygenase 2 Inhibitors;
Cyclooxygenase 2*;
Drug Therapy;
Head and Neck Neoplasms;
Heterografts*;
Humans*;
Mice;
Mice, Nude*;
Mouth*;
Radiation Tolerance;
Radiation-Sensitizing Agents;
Thigh;
Tumor Burden
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2006;49(4):420-425
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: It is known that tumor cells over-expressing COX-2 have resistance to many anticancer treatments. Among those treatments, along with surgery and chemotherapy, radiation therapy plays a significant role in the treatment of head and neck cancer. However, radiosensitivity of each cancer varies according to cancer types. Especially, the cancer over-expressing COX-2 is reported to have higher radioresistance to radiation therapy. The purpose of this study is to evaluate the effect of selective COX-2 inhibitor when combined with the radiation therapy, and to assess the possibility of clinical application of the selective COX-2 inhibitor for radiation therapy in the head and neck cancer. MATERIALS AND METHOD: The human oral cavity squamous carcinoma cells were cultured and xenografted in 40 athymic nude mice (1 x 10(7), left thigh, subcutaneous injection) and the mice were divided into 4 groups: the control group (10 mice), the radiation therapy group (10 mice, Group A), the Meloxicam injection group (10 mice, Group B), and the combination therapy group with radiation and Meloxicam (10 mice, Group C). The tumor volume was measured on every five days during the treatment and the tumor specimen was taken for immunohistochemical staining when the treatment was finished. The mean tumor volume, the apoptosis index and the proliferation index were measured. RESULTS: In the combination therapy group (Group C), the tumor growth rate was decreased compared to the radiation therapy group (Group A). Also, according to the result of the apoptosis index and the proliferation index measured using immunohistochemical staining, the combination therapy group presented a higher apoptosis index but a lower proliferation index than other groups. CONCLUSION: Meloxicam, selective COX-2 inhibitor, improves the efficacy of the radiation therapy for the human oral cavity squamous carcinoma and this effect was due to apoptosis modulation by selective COX-2 inhibitor.
