Loss of Heterozygosity on Chromosome 3p, 9p, 17p in Oral & Oropharyngeal Squamous Cell Carcinoma.
- Author:
Shi Hyung LEE
1
;
Min Sik KIM
;
Dong Il SUN
;
Chi Joon SONG
;
Seung Ho CHO
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea. entkms@cmc.cuk.ac.kr
- Publication Type:Original Article
- Keywords:
Oral cavity, Squarnous cell carcinoma;
Loss ofheterozygosip;
p16;
p53;
FHIT
- MeSH:
Biology;
Carcinoma, Squamous Cell*;
Chromosomes, Human, Pair 17;
Chromosomes, Human, Pair 3;
Chromosomes, Human, Pair 9;
DNA;
Genes, p16;
Genes, p53;
Genes, Tumor Suppressor;
Humans;
Loss of Heterozygosity*;
Lymph Nodes;
Microsatellite Repeats;
Neoplasm Metastasis;
Paraffin;
Polymerase Chain Reaction;
Recurrence
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2000;43(7):746-750
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Patients of oral and oropharyngeal squamous cell carcinoma in the comparable stage have diverse clinical courses and responses to similar treatment. Understanding the genetic alterations that occur in these neoplasms may help us understan4 their biology and behavior. Loss of heterozygosity (I.OH) correlates with inactivated tumor suppressor genes, and tumor suppressor genes are important in the development of oral and oropharyngeal squamous cell carcinoma. We invested LOH for the p53, p16, FHlT gene loci by PCR using microsatellite markers. MATERIALS AND METHODS: Specimens from 25 patients with oral and oropharngeal sqamous cell carcinomas were microdissected, and DNA was obtained from normal and tumoral tissues from paraffin embedded sections. PCR analysis was done with three primers, TP53 (17p13.1) for p53 gene and D9S165 (9p21) for p16 gene and D3S1067 (3p21.1-3p14.2) for FHIT gene. LOH was defined as reduction in the allelic ratio over 50%. RESULTS: LOH at the p53 locus was found in 6/17 of the informative cases (35%), and for the p16 locus, LOH was found in 4/13 of the informative cases (31%). For FHIT locus, LOH was found in 4/15 of the informative cases (27%). CONCLUSION: LOH on chromosome 3 (FHlT gene), chromosome 9 (p16 gene) and chromosome 17 (p53 gene) occurs in oral and oropharyngeal squamous cell carcinoma with high frequency suggesting that these tumor suppressor genes are involved in the development and progression of these carcinomas. There was no correlation behveen genetic alterations, age, pathologic stage, depth of invasion, cervical lymph node metastasis, recurrence and differentiation.
