Bone Resorption of Cultured Middle Ear Cholesteatomatous Epithelium-In Vitro Study.
- Author:
Hee Jong CHANG
1
;
Jong Woo CHUNG
;
Sang Hoon LEE
;
Young CHANG
;
Hyung Jong KIM
;
Tae Hyun YOON
Author Information
1. Department of Otolaryngology, University of Ulsan College of Medicine, Ulsan University Hospital, Ulsan, Korea. thyoon@www.amc.seoul.kr
- Publication Type:Original Article ; In Vitro
- Keywords:
Bone resorption;
Fetal rat calvarial culture;
Cholesteatoma;
Calcium release
- MeSH:
Animals;
Bone Resorption*;
Calcium;
Cholesteatoma;
Ear, Middle*;
Epithelial Cells;
Fibroblasts;
Injections, Subcutaneous;
Keratinocytes;
Otitis Media;
Rats;
Skin
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1998;41(9):1118-1121
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Bone resorption is an important cause of complications of chronic cholesteatomatous otitis media. Authors cultured the calvarial bone of fetal rat and measured the calcium release by keratinocytes and fibroblasts from cholesteatoma and postauricular skin (PAS). MATERIALS AND METHODS: Keratinocytes and subepithelial fibroblasts were cultured from cholesteatoma and PAS. The pregnant rats were sacrificed 24 hours after subcutaneous injection of Ca45 and the fetal calvarial bones were cultured with or without the supernatants of keratinocytes and fibroblast culture. The amount of released radioactive calcium was analyzed using beta-ray counter. RESULTS: The percentage (%) of calcium release was 28.95+/-4.0% in the control and 31.86+/-3.0% in the supernatant of PAS keratinocytes. In the cholesteatomatous keratinocytes, the released calcium was 34.99+/-6.1% and significantly greater than the values of control and PAS. Using the supernatants from the fibroblast culture, cholesteatoma and PAS showed higher calcium release than the control, but there was no significant difference between the two tissues. CONCLUSIONS: Through this study, authors showed the in-vitro bone resorption by cholesteatoma and concluded that cholesteatomatous epithelial cells can be involved in the bone resorption.
