Role of Hepatocyte Growth Factor and c-met Gene Expression in Oral Cavity and Oropharyngeal Squamous Cell Carcinoma.
- Author:
Jin Hwan KIM
1
;
So Jung OH
;
Young Soo RHO
;
Hwoe Young AHN
;
Hyung Sik SHIN
;
Seong Jin CHO
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Hallym University, Seoul, Korea. ys20805@chol.com
- Publication Type:Original Article
- Keywords:
Oral cavity;
Oropharynx;
Squamous cell carcinoma;
Hepatocyte growth factor;
Proto-oncogene c-Met proteins
- MeSH:
Carcinogenesis;
Carcinoma, Squamous Cell*;
Gene Expression*;
Head;
Hepatocyte Growth Factor*;
Hepatocytes*;
Immunohistochemistry;
Mouth*;
Neck;
Oropharynx;
Polymerase Chain Reaction;
Reverse Transcription;
RNA, Messenger;
Signal Transduction
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2007;50(8):684-689
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: The hepatocyte growth factor (HGF)/c-Met signal pathway may play various roles in carcinogenesis of several organs. However, studies about this pathway in head and neck cancers, especially oral cavity and oropharyngeal squamous cell carcinoma (SCC), are very rare. Our objectives are to evaluate the relationship between the mRNA and protein expression of HGF and c-met genes in oral cavity and oropharyngeal carcinomas. SUBJECTS AND METHOD: In this study, we examined the mRNA expression of HGF and c-Met by means of the immunohistochemistry (IHC) method and reverse transcription polymerase chain reaction (RT-PCR) in 40 cases of surgically resected oral cavity and oropharyngeal SCC and 10 cases of low grade dysplasia. RESULTS: Using RT-PCR, HGF mRNA amplification was detected in 67.5% and 10% of carcinoma and dysplasia. c-Met mRNA over-expression was detected in 57.5% and 20% of carcinoma and dysplasia. Using IHC, HGF and c-Met protein over-expression was detected in 55% and 62.5% in carcinoma, but not detected in dysplasia. CONCLUSION: These results suggest that HGF/c-Met signal pathway may be associated with the development of oral cavity and oropharyngeal SCC.
