Development of porcine respiratory and reproductive syndrome virus replicon vector for foot-and-mouth disease vaccine.
10.7774/cevr.2014.3.1.100
- Author:
Subbiah JEEVA
1
;
Jung Ah LEE
;
Seung Yong PARK
;
Chang Seon SONG
;
In Soo CHOI
;
Joong Bok LEE
Author Information
1. College of Veterinary Medicine and Veterinary Science Research institute, Konkuk University, Seoul, Korea. virus@konkuk.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Replicon;
FMDV;
PRRSV;
Genetic vectors
- MeSH:
Animals;
Antibodies;
Cercopithecus aethiops;
Clone Cells;
Coinfection;
Disease Outbreaks;
Electroporation;
Epithelial Cells;
Fluorescent Antibody Technique;
Foot-and-Mouth Disease*;
Genetic Vectors;
Genome;
Kidney;
Porcine respiratory and reproductive syndrome virus*;
Replicon*;
Sequence Analysis;
Transfection;
Viruses
- From:Clinical and Experimental Vaccine Research
2014;3(1):100-109
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Foot-and-mouth disease (FMD) is an economically important global animal disease. To control FMD virus (FMDV) outbreaks, a lot of different novel approaches have been attempted. In this study, we proposed a novel porcine reproductive and respiratory syndrome virus (PRRSV) as a replicon vector to express FMDV structural protein. MATERIALS AND METHODS: PRRSV infectious clone (PRRSVK418DM) was used to develop an expression vector through the reverse genetic manipulation of PRRSV; FMDVP12A3C gene of serotype O was synthesized and used for an antigen. MARC-145 cells (African green monkey kidney epithelial cell line) were used for electroporation mediated transfection. The transfection or the expression of P12A3C and N protein of PRRSV was analyzed by either replicon containing PRRSV alone or by co-infection of helper PRRSV. RESULTS: We constructed PRRSVK418DM replicon vector containing FMDVP12A3C, and genome sequences were confirmed by subsequent sequence analysis. In vitro expression of P12A3C and PRRSV N protein was confirmed by immunofluorescence antibody assay using antibodies specific for PRRSV N protein (anti-PRRSV N MAb), FMDV-VP1 (anti-VP1 MAb). CONCLUSION: The results indicate that PRRSV replicon vector can be a promising novel vector system to control FMDV and useful for vaccine development in the future.
