Comparison of Human and Avian influenza virus agglutinahility to 4 kinds of animal red blood cells
- VernacularTitle:Comparison of Human and Avian influenza virus agglutinahility to 4 kinds of animal red blood cells
- Author:
Narabold.N
;
Darmaa.B
;
Tsogtsaihan.S
- Publication Type:journal article
- From:Innovation
2009;6(1):77-77
- CountryMongolia
- Language:English
-
Abstract:
BACKGROUND
Every year 3-5 million cases have reported during human influenza epidemics and Disease 300 000-500 000 of them die. People who aged over 65 and under 2 year counted as a 'School of BioMeJicine. Health Sciences high risk group to influenza.
When mix red blood cells and influenza virus, it shows llemaglutination reaction,
which is caused by binding of influenza virus surface molecule hemagglutinin with red blood cell (RBC) receptor with syalic acid residues. This reaction is used traditionally as main and effective assay fw infl*wn*a virus detection.
PURPOSE
• To test local strains of A(H1N1), A(H3N2), A(II5N1) and B type influenza virus with human, guinea pig, chicken and horse RBCs
• To choose the most sensitive animal RBC for detection of different influenza viruses
METHODS
We have used isolates of A/Ulaanbaatar/1 N85/07 (H,N,), /J| i laanbaatar 1654 II (H,N,), B/Ulaanbaalar/1877/07 isolated in the Respiratory Virology Laboratory,
National Center for Communicable Diseases, A/Wooper Swan Mongolia 65 <16 (H5\ I)
isolated in Central Veterinary Laboratory with human 01. horse, chicken and guinea pig RBCs. The subtyping of the Human influenza viruses have been performed with rt-PCR with specific primers.
We used protocol of CDC, USA to isolate human influenza strains in MIX K cell culture, to determine strains with Hemagglutination test.
RESULTS
Human Influenza A(H IN I ).A(H3N2)and B type viruses are more agglutinable with to guinea pig RBCs
Avian influenza A(H5N1) virus is more agglutinable with human 01 and horse RBC.
CONCLUSIONS
Guinea pig RBCs is the most effective for daily human influenza virus detection procedures and using horse and guinea pig RBC together is reasonable tor avian influenza suspected cases.
