Comparison of the Toxoplasma gondii mice and cell culture derived antigens in ELISA assay
- Author:
Poshtehban, A., Shojaee, S.
;
Keshavarz, H., Salimi, M.
;
Mohebali, M.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2017;34(2):433-436
- CountryMalaysia
- Language:English
-
Abstract:
Abstract. Toxoplasmosis is an infectious disease caused by the coccidian parasite Toxoplasma
gondii. Diagnosis is based on serological methods with detection of specific IgG and IgM
antibodies. The present study was performed to compare the sensitivity and specificity of
soluble antigen of T. gondii, RH strain obtained from mice and cell culture in ELISA method.
Tachyzoites of T. gondii, RH strain that inoculated in mice peritoneum were collected. At the
same time, tachyzoites were harvested from HeLa cell culture that infected with the parasite.
Soluble antigen was prepared and ELISA method performed on 100 serum samples that were
collected from different laboratories in Tehran, Iran. Commercial Trinity kit was used as gold
standard. The sensitivity and specificity of T.gondii soluble antigen were higher in antigens
that obtained from cell culture in comparison with mice peritoneum. T. gondii cell culture
derived antigen has high sensitivity and specificity in ELISA test.
- Full text:P020170616550362335698.pdf
