Molecular detection of Anaplasma in apparently healthy Cholistan breed of cattle from the Bahawalpur district, Pakistan
- Author:
Hussain, M.F.
;
Qamar, M.
;
Malik, M.I.
;
Hussain, M.
;
Saeed, Z.
;
Shaikh, R.S.
- Publication Type:Journal Article
- From:Tropical Biomedicine
2017;34(1):37-44
- CountryMalaysia
- Language:English
-
Abstract:
The present study was designed to report the prevalence of Anaplasma sp. in blood
samples of Cholistan breed of cattle from Bahawalpur District and to determine the risk factors
associated with the prevalence of this parasite. A total of 148 blood samples were randomly
collected from apparently healthy cattle. On the sampling sites, data on the characteristics of
the animals (species, gender, age) were collected through questionnaires. 47 blood samples
(31.8% of total) produced the 577 base pairs DNA fragment specific for 16S rRNA gene of
Anaplasma sp. by PCR amplification. Out of 47 Anaplasma sp. positive PCR products, 9 were
found to be Anaplasma marginale by restriction with BssNa1 and 9 were confirmed to be
Anaplasma phagocytophilum (A. phagocytophilum) as they amplified 550 bp fragment from
the amplified MSP 2 gene of this species. Risk factor analysis indicated that the presence of
parasite was not limited to a particular sex or age group of the infected animals. Comparison of
hematological profile revealed that Anaplasma sp. positive cattle had significantly reduced
levels of mean corpuscular volume (P=0.02) and eosinophils (P=0.02) than in parasite negative
animals. While studied serum biochemical profile remain unaffected when compared between
the two groups.
- Full text:P020170529356016365918.pdf
