Effect of Mycoplasma pneumoniae on IL-8 Gene Expression in Human Respiratory Epithelial Cells.
- Author:
Myung Hyun SOHN
1
;
Kyung Eun LEE
;
Sung Yeon CHOI
;
Byung Chul KWON
;
Woo Kyung KIM
;
Gwang Cheon JANG
;
Myung Woong CHANG
;
Kyu Earn KIM
Author Information
1. Department of Pediatrics, Yonsei University College of Medicine, Korea. kekim@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Mycoplasma pneumoniae;
Interleukin-8;
Respiratory epithelial cells
- MeSH:
Asthma;
Blotting, Western;
Epithelial Cells*;
Gene Expression*;
Humans*;
Inflammation;
Interleukin-8*;
Lung;
Mitogen-Activated Protein Kinases;
Mycoplasma pneumoniae*;
Mycoplasma*;
Phosphorylation;
Phosphotransferases;
Pneumonia;
Pneumonia, Mycoplasma*
- From:Pediatric Allergy and Respiratory Disease
2004;14(1):38-45
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Mycoplasma pneumoniae is a common cause of lower respiratory disease. Several studies have suggested that M. pneumoniae respiratory infection is associated with reactive airway disease and asthma. It has been suggested that Interleukin-8 (IL-8) has a role in the pathogenesis of allergic inflammation of bronchial asthma and is well known to be expressed in bronchial epithelial cells. METHODS: In this study, we examined the effect of M. pneumoniae extract and the role of mitogen-activated protein kinases (MAPKs) on IL-8 expression in human lung epithelial cells. IL-8 levels were determined by an enzyme linked-immunosorbent assay. Extracellular signal-regulated kinase (ERK) phosphorylation was assessed by western blotting. RESULTS: In A549 cells, M. pneumoniae extract induced IL-8 release in a time- or dose- dependent manner. Pretreatment with PD98059, which blocks the activation of MAPK/ERK kinase 1, before M. pneumoniae extract stimulation inhibited the increase in the production of IL-8 by 64.4% at 25 M. Stimulation of A549 cells by extract caused an increase in the activity of ERK, compared with the non-stimulated cells. The extract stimulation had no effect on the activities of p38. CONCLUSION: These observations suggest that activation of ERK by M. pneumoniae extract may be one of the mechanisms that result in the increase of the production of IL-8.
