Sequence Analysis of ITS2 and CO1 Genes of Paragonimus harinasutai
- VernacularTitle:哈氏并殖吸虫ITS2基因和CO1基因序列分析
- Publication Type:Journal Article
- Keywords: Paragonimus harinasutai; Classification; Sequence analysis
- From: Chinese Journal of Parasitology and Parasitic Diseases 1987;0(02):-
- CountryChina
- Language:Chinese
- Abstract: Objective To identify Paragonimus harinasutai from Ninghai,Zhejiang Province,China.Methods Meta-cercariae were collected from the crabs Sinopotamon chekiangenes in Xixi village of Ninghai County for ITS2 sequence analysis,CO1 sequence analysis and endonuclease BsaHI and StuI analysis by PCR-RFLP.Results The fingerprintings of PCR-RFLP were virtually same to the isolate from Thailand(Nakorn-nayok).The ITS2 sequence with 366 bp and CO1 sequence with 390 bp of the metacercariae collected from Ninghai revealed a nucleotide identity 95.6% and 89.5% respectively to the Thai isolate.Conclusion The study confirmed that Paragonimus harinasutai is present in Ninghai,China,with certain variation on molecular biology in comparison to the Thai isolate.