The Screening of the Marine Low Temperature Acid Protease Strainfrom Pseudomonas and Its Optimal Fermentation Medium(I)
- VernacularTitle:海洋低温蛋白酶菌株选育及发酵培养基的研究(I)
- Author:
Naiyu CHI
- Publication Type:Journal Article
- Keywords:
Pseudomonas alcaligenes,Marine low temperature acid protease,Screening
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
Two Marine low temperature acid protease producing strains were obtained from more than 400 Pseudomonas,which isolated from marine.A marine low temperature acid protease producing mutant(Pa040523)was bred through multiple mutagenesis(UV,DES and NTG etc).Through orthogonal experiment,the optimal condition was given for producing marine low temperature acid protease of Pa040523.The suitable medium was that corn syrup,1.8 %;urea,0.6%;K_(2)HPO_(4),0.6%;KH_(2)PO_(4),0.3 %.The production of marine low temperature acid protease was 940.8 U/mg.
