A STUDY ON ULTRA LOW TEMPERATURE FREEZING OF ALKALOPHILIC MICROORGANISMS
- VernacularTitle:嗜碱细菌的液氮超低温冻结保藏
- Author:
Yuguang ZHOU
- Publication Type:Journal Article
- Keywords:
Ultro-low temperature freezing;
Alkalophilic microorganisms;
Protective agent
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
In this paper, response of the 7 bacterial strains belonging to alkalophilic microorganisms subjected to liquid nitrogen freezing and thawing was reported. According to the cell survival rate, the protective efficiency of 10% glycerol and 5% DMSO was equal to the protective efficency of the same method used in general bacterial cultures after preservation at frozen state for 3 months. The cell survival rate of alkalophilic microoraganisms should be increased if using alkaline protective agent.
