Preventative effect of melatonin on airway oxidative stress in rats with chronic obstructive pulmonary disease
- VernacularTitle:褪黑素对慢性阻塞性肺疾病大鼠气道氧化应激的预防作用
- Author:
Junwei XIA
- Publication Type:Journal Article
- Keywords:
Pulmonary disease;
Chronic obstructive;
Oxidative stress;
Melatonin
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of melatonin(MLT) on airway oxidative stress in rats with chronic obstructive pulmonary disease(COPD).Methods:36 male wistar rats were randomly divided into 3 groups as follows:control group,COPD group and MLT group.The COPD model was established by intratracheal lipopolysaccharide(LPS) and exposure to cigarette smoking for 28 days,the MLT group was given intraperitoneal injection of 10 mg/kg MLT 30 minutes before each cigarette challenge.After incision of trachea,the lung function was measured.Then the rats were lavaged,the total and different white blood cell counts of bronchial alveolar lavage fluid(BALF) were determined;nitric oxide(NO),malondialdehyde(MDA),superoxide dismutase(T-SOD),glutathione superoxide(GSH-PX) were carried out respectively.The pathological changes were observed under light microscope.Result :(1)Significant decrease in total white blood cells and neutrophils in BALF was found in MLT group than these in COPD group(P
