Inhibition effect of silencing EGFR gene by RNAi on tumorigenesis of xenograft tumor of ovarian cancer
- VernacularTitle:RNAi沉默EGFR基因在卵巢癌裸鼠移植瘤的成瘤抑制作用
- Author:
Yuanhong LIN
- Publication Type:Journal Article
- Keywords:
IEGFRgene;
RNAinterference;
Ovariancancer
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the inhibition effect of silencing EGFR gene by RNAi on tumorigenesis of ovarian cancer SKOV3 cells. Methods: The EGFR gene sequence-specific dsRNA expression vector was constructed,lipofectamine transfer SKOV3 cells and G418 were used for the selection of positive clones. Xenograft tumor models were established.The model mice were divided into positive interference group,negative control group,blank control group. The tumor volume was monitored;the tumor quality was measured;the expression of EGFR gene in the xenograft tumor was detected by RT-PCR,Western Blot and immunohistochemical. Results: The tumorigenesis of the positive interference group decreased significantly,EGFR mRNA and protein inhibition rates were 78.8% and 76.4%,respectively. Conclusion: The animal experiment was confirmed that Silencing of EGFR gene by RNAi can obviously inhibit the tumorigenesis of ovarian cancer.
