Effects of ischemic reperfusion on the expression of MCP-1 and HO-1 and the cardio-protective role of erythropoietin
- VernacularTitle:缺血再灌注对大鼠心脏MCP-1和HO-1表达的影响及促红细胞生成素的心肌保护作用
- Author:
Xifu CHENG
- Publication Type:Journal Article
- Keywords:
Ischemia-reperfusion;
Myocardial ischemia-reperfusion injury;
Erythropoietin;
Monocyte chemoattractant protein-1;
Heme oxygenase-1
- From:
Journal of Chongqing Medical University
2007;0(11):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To investigate the effect of ischemic reperfusion(I-R) on the expression of Monocyte chemoattractant protein-1(MCP-1)and heme oxygenase-1 protein(HO-1) and mRNA of myocardial tissue and the protective role of erythropoi- etin (EPO). Methods:Ischemic reperfusion rat model was established in the experiments.24 animals were randomly divided into sham operation(F),I-R group(O)and EPO group(E)and subjected to 45 min of myocardial ischemia by ligation of left anteri- or descending coronary artery followed by 180 min of reperfusion.The animals in EPO group were intraperitoneal by injec- tioned with EPO(4 000 U/Kg)24 hours before the operation. The expression of MCP-1 and HO-1 protein and mRNA was de- termined by immunohistochemistry and RT-PCR. Results:Campared with F group, the expression of myocardial MCP-1 and HO-1 protein and mRNA increased significantly in O group and E group (P
