Study on Partial Biochemical Characterization and Effect of Lumbricus Dornases
- VernacularTitle:地龙脱氧核糖核酸酶的部分性质研究及作用初探
- Publication Type:Journal Article
- Keywords: lumbricus; dornases; molecular weight; pI
- From: Chinese Journal of Information on Traditional Chinese Medicine 2006;0(03):-
- CountryChina
- Language:Chinese
- Abstract: Objective To study partial biochemical characterization and effect of lumbricus dornases (LDs). Methods Sephacryl-200 column chromatography, CIEF and agarose electrophoresis were used to determine LDs. Results The molecular weights of LD1 and LD2 were 126 kDa and 62 kDa respectively. The pI of LD1 and LD2 were 6.12 and 6.05. LDs degraded bacterial plasmid and ?DNA. Conclusion LDs is a new kind of dornases. The determination of molecular weights and pI of LDs will help their further study. The results of plasmid and ?DNA degradation imply that LDs can oppose bacteria drug resistance.