Determination of Ursolic Acid in Erlongzuoci Pills by HPLC-ELSD
- VernacularTitle:HPLC-ELSD法测定耳聋左慈丸中熊果酸的含量
- Author:
Xianmei YAO
- Publication Type:Journal Article
- Keywords:
Erlongzuoci pills;
Ursolic acid;
HPLC-ELSD
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The method for determination of ursolic acid in Erlongzuoci pills by HPLC-ELSD was established. Methods VP-ODS C18 column was used with the mobile phase of methanol-0.5% acetic acid (90∶10). The flow rate was 0.8 mL/min. Results Ursolic acid showed a good linear relationship at the range of 63~630 ?g/mL (r=0.999 9). The average recovery was 98.11% (RSD=1.14%). Conclusions The method is simple,accurate and reproducible. It can be used for determination of ursolic acid in Erlongzuoci pills.