Expression of pituitary tumor-transforming gene in endometrial carcinoma
- VernacularTitle:子宫内膜癌组织中垂体肿瘤转化基因PTTG的表达及其意义
- Publication Type:Journal Article
- Keywords: Neoplasm proteins; Endometrial neoplasms; Fibroblast growth factor 2; Neovascularization, pathologic; Immunohistochemistry; Semi-quantitive reverse; Polymerase chain reaction
- From: Chinese Journal of Obstetrics and Gynecology 2001;0(08):-
- CountryChina
- Language:Chinese
- Abstract: Objective To study the expression of pituitary tumor-transforming gene (PTTG) and its relationship with the expression of basic fibroblast growth factor ( bFGF) protein and microvessel density (MVD) in endometrial carcinoma. Methods Expressions of PTTG mRNA and protein were assessed by semi-quantitive RT-PCR and immunohistochemistry methods respectively in 50 cases of endometrial carcinomas, 15 cases of hyperplasia endometria and 12 cases of normal endometrial tissue. Expressions of bFGF protein were detected by immunohistochemistry. Microvessels were highlighted by staining endothelial cells with CD34 antigen, and MVDs were counted Results The expression rate and average quantity of PTTG mRNA were detected in a significantly greater proportion endometrial carcinomas (96% ,0. 84 ?0. 08) than in hyperplasia endometria (60% ,0.78 ? 0.06) and normal endometrial tissue (33% ,0.48 ? 0. 12, P 0. 05, respectively). The average quantity of PTTG mRNA and expression rate of PTTG protein in tissues with bFGF protein coexpression(0. 86 ? 0.07,87%) were higher than in those without bFGF protein coexpression (0. 80 ? 0. 06, 42% , P