Establishment of a hyperinsulinemic-euglycemic clamp technique in conscious rats
- VernacularTitle:清醒状态大鼠高胰岛素-正常血糖钳夹术的建立及其意义
- Author:
Sichuan LUO
- Publication Type:Journal Article
- Keywords:
Hyperinsulinemic-euglycemic clamp;
Insulin sensitivity;
Lipid;
Anesthesia
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a hyperinsulinemic euglycemic clamp technique in conscious rat,and to explore the effect of anesthesia and acute infusion of lipid on insulin sensitivity of rats.Methods:Rats divided into two groups were catheterized in the right jugular vein and left carotid artery and underwent a hyperinsulinemic-euglycemic clamp with or without infusion of lipid (Intralipid) and in unconscious and conscious state.Results:Infusion of lipid resulted in a decrease of insulin sensitivity by 30%( P
