Effect of [K+] on corneal endothelial cell survival
- VernacularTitle:高钾保存液对角膜内皮细胞存活的影响
- Author:
Chn OPHTHALMOL
- Publication Type:Journal Article
- Keywords:
Endothelium corneal;
Potasslum;
Flow cytometry
- From:
Ophthalmology in China
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
A dual-color flow cytometric assay (two dyes: cyanine membrane dye-3, 3'-Dioctadecyloxaearboeyanine perehlorate and propidium iodide) was used for the assessment of bovine corneal endothelial cell survival in potassium-rich solution and K medium (prepared by Kaufman method) for 3, 5, 7, 9, and 11 days at 4℃. Results showed that (1) the rate of survivable endothelial cells stored in potassium-rich solution was significantly higher than that in K medium for 5, 7, 9, and 11 days respectively (P
