A STUDY ON HBV DNA IN PATIENTS WITH SERUM HBsAg-NEGATIVE AND POSITIVE CHRONIC ACTIVE HEPATITIS
- VernacularTitle:血清HBsAg阴性和HBsAg阳性慢性活动性肝炎患者肝内HBV DNA的研究
- Publication Type:Journal Article
- Keywords: Hepatitis B Chronic active hepatitis HBV DNA HBcAg HBsAg
- From: Medical Journal of Chinese People's Liberation Army 1981;0(06):-
- CountryChina
- Language:Chinese
- Abstract: HBV DNA was detected by in situ hybridization using a biotin labelled probe and HBV antigens (including HBsAg and HBcAg) and by avdin-biotin-peroxidase complex technique in liver sections from 34 patients negative for serum HBsAg and 30 patients positive for HBsAg of chronic active hepatitis.The incidence of intrahepatic HBV DNA,HBcAg and HBsAg in 34 patients negative for serum HBsAg were 23.5%,23.5% and 32.4%,and that in 30 patients positive for serum HBsAg were 63.3%,56.7% and 83.3%,respectively.In a few patients negative for HBsAg,though positive for HBV antibody (even for anti-HBs) or negative for HBV antiboody,HBV DNA and HBV antigens could still be detected in liver tissue.In these patients who showed positive HBsAg,no matter positive for serum HBeAg,or positive for anti-HBe,or negative for HBeAg,all of them had high incidence of HBV DNA positivity in liver,which were 68.8%,60.0% and 55.5%,respectively.Intrahepatic HBcAg was associated closely with the presence of HBV DNA in hepatocytes,suggesting that it could be considered as an index to reflect HBV DNA synthesis.