Detection of Spoilage Bacteria in Beer by Polymerase Chain
- VernacularTitle:聚合酶链式反应快速鉴定啤酒有害菌研究
- Author:
Xiaoqun TIAN
- Publication Type:Journal Article
- Keywords:
Beer spoilage,PCR,Rapid detection,Sensitivity
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
A new method for rapid detecting beer-spoilage by PCR was developed.This method is based on the sequence of a hop-resistance gene horA,which a pair of specific primers were designed based on the partial sequence of it.Using the primers,beer-spoilage Lactobacillus species were detected by PCR.The sensitivity was reached to 3CFU.The pre-enrichment of samples was needed only 12~16 h.The duration for beer-spoilage determination by PCR(24h)was markly shorter than by conventional method(5~7d).
