Combined Effects of Lead and Cadmium on NAG of Rats' Renal Tubular Epithelial Cells WANG
- VernacularTitle:铅镉联合作用对大鼠肾小管上皮细胞N-乙酰-?-D-氨基葡萄糖苷酶的影响
- Publication Type:Journal Article
- Keywords: Lead; Cadmium; Renal tubular epithelial cells; N-acetyl-?-D-glucosaminidase
- From: Journal of Environment and Health 1992;0(04):-
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the combined effects of lead and cadmium on N-acetyl-?-D-glucosaminidase (NAG) of rats' renal tubular epithelial cells. Methods After extraction, purification, identification and culturing, renal tubular epithelial cells of rats were treated with lead acetate of 0, 0.02, 0. 1, 0.5 mmol/L, cadmium chloride of 0, 0.001, 0.004, 0.02 mmol/ L, and lead-cadmium of 2x2 pattern. NAG leaking out of cells was assessed. Results When cells were solely treated with lead acetate of 0.02 and 0. 1 mmol/ L, NAG activities in experimental groups showed no significant difference compared with that in the control group, but showed significant difference with a dosage of 0.5 mmol/L. When cells were solely treated with cadmium chloride of 0.001 and 0. 004mmol/L, NAG also showed no significant difference compared with that in the control group, while showing a significant difference with a dosage of 0.02 mmol/L. When cells were treated jointly with lead-cadmium of different dosages, NAG was significantly higher than that in the experimental groups treated only with lead or cadmium. Conclusion The combined effects of lead and cadmium led to more NAG leaking out of renal tubular epithelial cells of rats.