EVALUATION OF THE ASSAYS FOR THYROID-STIMULATING ANTIBODY MEASURING ~(125)I-UPTAKE AND cAMP RELEASE BY RAT THYROID CELLS
- VernacularTitle:鼠甲状腺细胞~(125)Ⅰ摄取法和cAMP释放法测定甲状腺刺激抗体的评价
- Author:
Shixindept JIN
- Publication Type:Journal Article
- Keywords:
Thyroid-stimulating antibody Rat thyroid cell 125I-uptake cAMP assay Hyperthyroidism
- From:
Chinese Journal of Endocrinology and Metabolism
1985;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
Thyroid-stimulating antibody (TSAb) was measured in 16 patients with active Graves' disease based on 125I-uptake-and cAMP release of rat thyroid cell strain FRTL5 according to Marcocci and Kasagi with modifications simultaneously. With the method of 126I-uptake, TSAb was found positive in 56% of the patients and the TSAb index was 1.19; with, the method of cAMP release, these two figures were 94% and 7.11 respectively. There were very significant differences for both. The method of cAMP release was sensitive and convenient:1) the cells may multiply continuously, the IgG-may be prepared easily,and the cAMP in the medium may be measured without extraction; 2) in 35 untreated and 42 treated patients with Graves' disease, the TSAb was positive in 91 % and 50% of the cases, and the TSAb activities were 1194% and 276% respectively, the differences were also very significant.
