THE APPLICATION OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY TO THE DIAGNOSIS AND CLASSIFICATION OF CONGENITAL ADRENAL HYPERPLASIA
- VernacularTitle:高效液相色谱法在先天性肾上腺增生症诊断和分型中的应用
- Author:
Jiqing WEI
- Publication Type:Journal Article
- From:
Chinese Journal of Endocrinology and Metabolism
1985;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
A high-performance liquid chromatographic procedure (HPLC) for separating and measuring cortisone (E), cottisol (F), corticosterone (B),11-deoxycortisol (S), 4-androstene-dione + 11-deoxycorticosterone (A + DOC), 17?-hydroxy-progesterone (17?-OHP), and progesterone (P) in a single serum sample was described. With this method, the steroids in 6 patients.with congenital adrenal hyperplasia (CAH) and 30 normal subjects were determined. In 3 untreated patients with CAH due to 21-hydroxylase deficiency (21-OHD), basal serum 17?-OHP, A + DOC, and 21-hydroxylase index were markedly elevated, whereas F and E were considerably reduced; there was a "tripeak" in the shape of stairs seen on their chromatograms. In a treated case of 21-OHD, the "tripeak" disappeared. In two girls with 17?-hydroxylase deficiency, basal serum B was very high whereas F and E were very low. Their 17?-hydroxylase index was more than 70 times that of the normals. The above mentioned results suggested the usefulness of HPLC to the diagnosis and classification of CAH.
