Determination of Concentration of Dextromethorphan Hydrobromide in Human Serum by HPLC
- VernacularTitle:高效液相色谱法测定人血清中氢溴酸右美沙芬浓度
- Author:
Fangchen PENG
- Publication Type:Journal Article
- Keywords:
HPLC;
dextromethorphan hydrobromide;
blood concentration
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for determination of concentration of dextromethorphan hydrobro?mide in human serum.METHODS:The assay was conducted on HiQsil C 18 column(4.6mm?150mm,5?m)with acetonitrile-water-acetic acid(35∶63∶2,V/V/V)as mobile phase.The fluorescence detector measured with the excitation wavelength of280nm and the emission wavelength of320nm,and carvedilol was taken as internal standard.RESULTS:The linear range of calibration curve of dextromethorphan hydrobromide was0.27～21.90ng/ml with a regressive equation of Y=0.1197X+0.0086,r=0.9986.The recovery was high.CONCLUSION:This method is accurate,rapid and simple,and can be used for de?termining the concentration of dextromethorphan hydrobromide in human serum.
