Determination of Ephedrine Hydrochloride and Diphenhydramine Hydrochloride in Baichuaipeng Tablets by HPLC
- VernacularTitle:HPLC法同时测定百喘朋中盐酸麻黄碱和盐酸苯海拉明的含量
- Author:
Huiyue CHENG
- Publication Type:Journal Article
- Keywords:
Baichuaipeng tablet;
ephedrine hydrochloride;
diphenhydramine hydrochloride;
HPLC
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for simultaneously detecting the contents of ephedrine hydrochloride and diphenhydramine hydrochloride in Baichuaipeng tablets METHODS:The conditions of detection were:Diamonsil C18 (4 6mm?150mm,5?),the mobile phase consisted of acetonitrile-water-phosphoric acid (60∶40∶0 1,each 1 000ml contained 4 0g sodium lauryl sulfate),flow-rate 1ml/min,detection wavelength 254nm,external standard method RESULTS:The good linearity between peak and quantity ranged form 2 69?g～32 28?g for ephedrine hydrochloride and 2 54?g～30 48?g for diphenhydramine hydrochloride(n=5) The average recoveries(n=5) of ephedrine hydrochloride and diphenhydramine hydrochloride were 100 4%(RSD=1 1%) and 99 8%(RSD=0 9%),respectively CONCLUSION:The method is accurate,sim_ple and suitable for the quality control of Baichuaipeng tablets
