A STUDY OF HUMAN MITOCHONDRIAL DNA POLYMORPHISM REGION SEQUENCING
- VernacularTitle:用PCR—测序法对人类线粒体DNA多态区的研究
- Author:
Chunbin ZHANG
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction (PCR) DNA sequencing Mitochondrial DNA (mtDNA)Polymorphism
- From:
Chinese Journal of Forensic Medicine
1987;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
By means of Polymerase chain reaction (PCR), the mitochondrial DNA polymorphous region from the base number 15997 to 16401 was amplified. After purification,the amplified fragment was directly Sequenced on automatic .DNA sequencer and accurate results was obtained. Compared with the former reports, there are base divergence between the results of this paper and sequence of Caucasian. Changes of base in six samples were observed.The numbers of base change are 3 to 6. The maternal inheritance of the mitochondrial genome makes mtDNA sequencing via PCR an attractive tool for testing whether individuals are maternally related. The method can also be used for studying of race inheritance and molecular evolution. The method is rather simpler and quicker than the former methods.
