Signifioant Influence of Sample Processing on the Assay of Adenine Nucleotides, Lactate and Pyruvate in Rat Muscle
- VernacularTitle:组织样品处理方法对肌肉腺苷酸、乳酸和丙酮酸测定值的影响
- Author:
Jiming YE
- Publication Type:Journal Article
- Keywords:
Adenine nucleotide Lactate;
Pyruvate Sample processing
- From:
Journal of Kunming Medical University
1989;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
An ideal assay of adenine nucleotides, lactate and pyruvate in the extract of rat freeze-clamped muscle was achieved. Electromicroscopy showed that the cell membrane was destroyed by powdering the muscle in liquid N_2 and then homogenizing for 1 min. An additien of ultrasonic treatment for 1 min could thoroughly break the subcellular structures and lead to a remarkable increase in ATP and total adenine nucleotides with a little change of lactate and pyruvate. The prolongation of the treatment for two more minutes, however, could result in the breakdown of ATP.
