Expressions of epithelial-mesenchymal transition-associated genes in cervical cancer cell lines
10.7652/jdyxb201702011
- VernacularTitle:宫颈癌细胞系中EMT相关基因的表达及其意义
- Author:
Xiaofeng LI
;
Wei CHEN
;
Xu LI
;
Le ZHAO
- Keywords:
cervical cancer;
epithelial-mesenchymal transition (EMT);
E-cadherin;
vimentin;
Snail;
Slug
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(2):210-214
- CountryChina
- Language:Chinese
-
Abstract:
Objective Epithelial-mesenchymal transition (EMT)is thought to be one of the important mechanisms of invasion and metastasis of carcinoma cells,which includes down-regulation of epithelial cell markers and up-regulation of mesenchymal markers.The aim of our study was to investigate whether EMT occurs in cervical cancer cell lines and determine the proper cell model to study the mechanism of EMT in cervical cancer.Methods We detected the mRNA expression of EMT-associated genes,namely,E-cadherin,vimentin,Snail and Slug,in four cervical cancer cell lines using reverse transcriptase-polymerase chain reaction analysis.E-cadherin and vimentin protein expression levels were examined using Western blotting. Snail and Slug protein expression levels were examined by immunocytochemical staining.Results Vimentin and Snail mRNA was expressed in all of the four cell lines.E-cadherin and Slug mRNA was expressed in SiHa,HeLa and RJC-1 cells except CS1213 cells.E-cadherin protein was found in SiHa and RJC-1 cells whereas vimentin protein was detected in HeLa and CS1213.Snail protein was expressed in all of the four cell lines.Slug protein was found in SiHa,HeLa and RJC-1 cells.Conclusion It seems that HeLa and CS1213 display the phenotype of mesenchymal cells whereas SiHa and RJC-1 remain the epithelial phenotype.
