Ethanol Modulates Mitochondrial Production of AC16 Cardiomyocytes by Interfering with PGC-1αExpression
10.12007/j.issn.0258-4646.2016.12.009
- VernacularTitle:乙醇通过干预PGC-1α表达水平调节AC16心肌细胞线粒体生成
- Keywords:
ethanol;
peroxisome proliferator-activated receptorγcoactivator-1α;
nuclear respiratory factor 1;
mitochondrial transcrip-tion factor A;
AC16 cardiomyocyte;
mitochondrial biogenesis
- From:
Journal of China Medical University
2016;45(12):1094-1099
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of peroxisome proliferator?activated receptorγcoactivator?1α(PGC?1α),nuclear respiratory factor 1(NRF?1),mitochondrial transcription factor A(TFAM)and mitochondrial structural and functional alterations under different concentra?tions of ethanol. Methods MTT assay was used to determine the survival rate of AC16 cells under different concentrations of ethanol,and the ap?propriate concentration of ethanol used for subsequent experiments was selected. Mitochondrial membrane potential of AC 16 cells was measured by flow cytometry using fluorescent probe JC?1. The ultrastructural changes of different groups of cells was observed under the electron microscope. The expressions of PGC?1αand its target proteins NRF?1 and TFAM were detected by real?time PCR and Western blotting. Results The number of mitochondrion,mitochondrial membrane potential,and the expressions of PGC?1α,NRF?1,and TFAM were increased in low?concentration eth?anol group(50 mmol/L). While in high?concentration ethanol group(200 mmol/L),they were all decreased,some mitochondria were larger than normal size;the cristae in some mitochondria were disordered,and some mitochondria had no cristae,appearing as circular or elongated vesicles. Conclusion The effect of ethanol on cardiovascular disease is mainly by regulating the expressions of PGC?1αand its downstream proteins NRF?1 and TFAM,which are the key factors in regulating mitochondrial biogenesis.
