Inhibition of Mahkota Dewa (Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E

Keywords: Phaleria macrocarpa; Mahkota Dewa; DLBS 1425; Retinoblastoma; Cyclin E; Cell cycle
From:Asian Pacific Journal of Tropical Biomedicine 2017;7(4):280-287
CountryChina
Language:Chinese
Abstract: Objecive:To prove the molecular mechanisms of Mahkota Dewa (Phaleria macrocarpa) in suppressing proliferation of human retinoblastoma cells through suppression of cell cycle's gene-rcgulators expression.Methods:In this study,the molecular mechanism of anti-tumor effect of fractioned extract of Phaleria macrocarpa (DLBS1425) in human retinoblastoma cells Y-79 was investigated by measuring the tumor cells viability,the assessment of population profiles of tumor cells in the cell cycle,and the mRNA concentration of pi6,p21,p53,cyclin D,cyclin E,and E2F.Results:DLBS1425 showed an inhibition effects towards proliferation of Y-79 cell line.Inhibition of proliferation was shown by suppression of cell cycle progression.DLBS1425 downregulated cyclin E,a G1 phase regulator gene of cell cycle,in dosedependent manner without affecting p53-p21 pathway.In the other word,DLBS1425 inhibits cell proliferation through suppression of cyclin E independently towards conventional proliferation pathway.Conclusions:Our results suggest that DLBS 1425 is a potential anticancer agent which targets genes involved in cell proliferation in human retinoblastoma cells which make it pharmacologically ideal for the prevention and/or treatment of retinoblastoma cancer.