Identification of antigenic proteins from salivary glands of female Anopheles maculatus by proteomic analysis
- Publication Type:Journal Article
- Keywords:
Saliva;
Immunogenic;
Proteins;
Anopheles
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(11):924-930
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify antigenic proteins from the salivary glands of female Anopheles maculatus using a proteomic approach to find the biomarker candidate for serological tools.
Methods: The identification of antigenic proteins of Anopheles maculatus salivary gland used these techniques: one-dimensional gel electrophoresis (sodium dodecyl sulfate polyacrylamide gel electrophoresis), western blot, and liquid chromatography–mass spectrometry.
Results: The proteins that have molecular weight (MW) 43 and 34 kDa were the anti-genic protein. Computational bioinformatic analysis by Mascot Server revealed seven novel hypothetical proteins (MW: 43 kDa) and two novel hypothetical proteins (MW:34 kDa). Further analysis (BLASTP, antigenicity, epitope mapping, and specificity analysis) showed that two novel proteins were identified as apolipoprotein D and cathepsin D in Anopheles darlingi.
Conclusions: The identified proteins are potential to be developed as a biomarker of mosquito bite's exposure.
